AGRIKOLA: Systematic RNAi knockouts in Arabidopsis

The AGRIKOLA project (Arabidopsis Genomic RNAi Knock-out Line Analysis) is funded by the European Union as part of the 5th Framework Programme, and started officially on the 1st November 2002. Six European labs are using high-throughput recombinational cloning to clone gene-specific tags (GSTs) into binary hairpin RNA vectors and to use many of the resulting plasmids to create a collection of silenced Arabidopsis lines. The GSTs used in this project were originally developed by the CATMA consortium, comprising plant genomics laboratories in eight European countries, for use in microarray studies.

The reference to cite when using data or resources from AGRIKOLA:

Hilson P, Allemeersch J, Altmann T, Aubourg S, Avon A, Beynon J, Bhalerao RP, Bitton F, Caboche M, Cannoot B, Chardakov V, Cognet-Holliger C, Colot V, Crowe M, Darimont C, Durinck S, Eickhoff H, Falcon de Longevialle A, Farmer EE, Grant M, Kuiper MTR, Lehrach H, Léon C, Leyva A, Lundeberg J, Lurin C, Moreau Y, Nietfeld W, Paz-Ares J, Reymond P, Rouzé P, Sandberg G, Dolores Segura M, Serizet C, Tabrett A, Taconnat L, Thareau V, Van Hummelen P, Vercruysse S, Vuylsteke M, Weingartner M, Weisbeek P J, Wirta V, Wittink FRA, Zabeau M and Small I (2004) Versatile gene-specific sequence tags for Arabidopsis functional genomics: transcript profiling and reverse genetics applications, Genome Research 14, 2176-2189.

AGRIKOLA resources are distributed by the Nottingham Arabidopsis Stock Center and can be ordered on line.

NEW! Purified sequence-validated AGRIKOLA clones can also be ordered on line via the Belgian BCCM/LMBP

This web site is hosted by Department of Plant Systems Biology -VIB, Ghent University.